Journal: Frontiers in Physiology
Article Title: Investigation of mitochondrial inner membrane ion conductance by planar lipid bilayer electrophysiology
doi: 10.3389/fphys.2026.1782998
Figure Lengend Snippet: Schematic representation of a planar lipid bilayer experiment. The experiments are performed in a recording chamber with a Derlin cuvette that has two compartments, cis and trans . These compartments are connected via a small aperture with a diameter of 50-250 μm. (a) The lipid mixture, dissolved in decane, is applied to the aperture using a fine paintbrush. (b, c) Upon subsequent formation of a planar lipid bilayer membrane on a cuvette aperture, IMVs or purified protein are added to the cis- compartment of the chamber. (d) The reconstitution of the added IMVs into the bilayer membrane is achieved by diffusion-led vesicle fusion under the influence of applied voltage or short voltage pulses.
Article Snippet: Planar lipid bilayer recordings were performed in a 13 mm Derlin cuvette (Warner Instruments) with a 200 μm aperture ( ).
Techniques: Membrane, Purification, Diffusion-based Assay